Product name
Recombinant Rat IL-1RA protein
Code
CD00109
Biological activity
Fully biologically active when compared to standard. The ED50 as determined by inhibiting IL-1α-dependent proliferation of Mouse D10S cells is less than 150 ng/ml, corresponding to a specific activity of > 6.7 × 103 IU/mg in the presence of 50 pg/ml rRtIL-1α.
Endotoxin
Less than 1 EU/μg of rRtIL-1RA as determined by LAL method.
Synonyms
IL-1RA, IL1RN,Rat
Sequence
HPAGKRPCKM QAFRIWDTNQ KTFYLRNNQL IAGYLQGPNT KLEEKIDMVP IDFRNVFLGI HGGKLCLSCV KSGDDTKLQL EEVNITDLNK NKEEDKRFTF IRSETGPTTS FESLACPGWF LCTTLEADHP VSLTNTPKEP CTVTKFYFQE DQ
Uniprot Accession
Molecular Weight
Approximately 17.4 kDa, a single non-glycosylated polypeptide chain containing 152 amino acids.
Formulation
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Background
IL-1RA was initially called the IL-1 inhibitor which is encoded by the IL1RN gene and it is a member of the interleukin 1 cytokine family. IL-1RA is secreted by various types of cells including immune cells, epithelial cells, and adipocytes. IL-RA has functions of inhibiting the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the co-receptor IL1RAP for signaling. IL-1RA is also used in the treatment of rheumatoid arthritis, an autoimmune disease in which IL-1 plays a key role. The rat IL-1RA is a single non-glycosylated polypeptide chain containing 152 amino acids and it has been shown to block the inflammatory responses induced by IL-1 both in vitro and in vivo. Rat IL-1RA shares 89 % and 73 % a.a. sequence homology with Mouse and human IL-1RA.
Storage
-20ºC
Research area
Immunology
