Product name
Recombinant Rat Fractalkine/CX3CL1 protein
Code
CD00123
Biological activity
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration of 5.0-10 ng/ml.
Endotoxin
Less than 1 EU/μg of rRtFractalkine/CX3CL1 as determined by LAL method.
Synonyms
Fractalkine/CX3CL1,Cx3c; Scyd1, Rat
Sequence
QHLGMTKCNI TCHKMTSPIP VTLLIHYQLN QESCGKRAII LETRQHRHFC ADPKEKWVQD AMKHLDHQTA ALTRNG
Uniprot Accession
Molecular Weight
Approximately 8.8 kDa, a single non-glycosylated polypeptide chain containing 76 amino acids and comprises only the chemokine domain of Rat Fractalkine.
Formulation
Lyophilized from a 0.2 μm filtered concentrated solution in 2× PBS, pH 7.4.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Background
CX3CL1 recently identified through bioinformatics is the only known member of the CX3C chemokine family and it is also commonly known under the names fractalkine (in humans) and neurotactin (in mice). Unlike other known chemokines, CX3CL1 is a type 1 membrane protein containing a chemokine domain tethered on a long mucinlike talk. The soluble form of CX3CL1 is chemotactic for T-cells and monocytes, but not for neutrophils. In addition, it may play a role in regulating leukocyte adhesion and migration processes at the endothelium. Recombinant Rat CX3CL1 contains 76 amino acids and it shares approximately 86 % and 83 % amino acid sequence homology with the Mouse and human protein.
Storage
-20ºC
Research area
Immunology
