Product name
Recombinant Mouse IL-21 protein
Code
CD00368
Biological activity
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human N1186 T cells is less than 25 ng/ml, corresponding to a specific activity of > 4.0 × 104 IU/mg.
Endotoxin
Less than 0.1 EU/μg of rMuIL-21 as determined by LAL method.
Synonyms
IL-21, IL21,Mouse
Sequence
HKSSPQGPDR LLIRLRHLID IVEQLKIYEN DLDPELLSAP QDVKGHCEHA AFACFQKAKL KPSNPGNNKT FIIDLVAQLR RRLPARRGGK KQKHIAKCPS CDSYEKRTPK EFLERLKWLL QKMIHQHLS
Uniprot Accession
Molecular Weight
Approximately 15.0 kDa, a single non-glycosylated polypeptide chain containing 129 amino acids.
Formulation
Lyophilized from a 0.2 μm filtered concentrated solution in 2 × PBS, pH 7.4.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 C. Further dilutions should be made in appropriate buffered solutions.
Background
Mouse IL-21 is a pleiotropic cytokine produced by CD4+ T cells in response to antigenic stimulation and can regulating immune system cells. It promotes the anti-tumor activity of CD8+ T cells and NK cells. IL-21 elicits its effect through binding IL-21R, which also contains the gamma chain found in other cytokine receptors such as IL-2, IL-4, IL-7, IL-9 and IL-15. The IL-21/IL-21R interaction triggers a cascade of events which includes activation of the tyrosine kinases JAK1 and JAK3, followed by activation of the transcription factors STAT1 and STAT3. IL-21 shows having much relation with clinical illnesses, including cancer immunotherapy, viral infections and allergies.
Storage
-20ºC
Research area
Immunology
