Product name
Recombinant Mouse IL-10 protein
Code
CD00393
Biological activity
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using Mouse MC/9-2 cells is less than 1 ng/ml, corresponding to a specific activity of > 1.0 × 106 IU/mg.
Endotoxin
Less than 1 EU/μg of rMuIL-10 as determined by LAL method.
Synonyms
IL-10,CSIF, IL-, If2a, Il10, Mouse
Sequence
SRGQYSREDN NCTHFPVGQS HMLLELRTAF SQVKTFFQTK DQLDNILLTD SLMQDFKGYL GCQALSEMIQ FYLVEVMPQA EKHGPEIKEH LNSLGEKLKT LRMRLRRCHR FLPCENKSKA VEQVKSDFNK LQDQGVYKAM NEFDIFINCI EAYMMIKMKS
Uniprot Accession
Molecular Weight
Approximately 18.7 kDa, a single non-glycosylated polypeptide chain containing 160 amino acids.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS, pH 7.4.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 10 mM HCl to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Background
Interleukin-10 (IL-10) is encoded by the IL10 gene and expressed in monocytes and Type 2 T helper cells (Th2). The biological activities of IL-10 are mediated by the IL-10 receptor complex, which is composed of the ligand-binding IL-10 Rα/R1 and the accessory IL-10 Rβ/R2 subunits. IL-10 is capable of inhibiting the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by T helper cells. IL-10 is classified as a class 2 cytokine which consist of IL-10, IL-19, IL-20, IL-22, IL-24 and IL-26, several interferons and interferon-like molecules. Mature Mouse IL-10 shares 70 %-77 % amino acid sequence identity with human, bovine, porcine IL-10, but Mouse IL-10 does not act on human cells.
Storage
-20ºC
Research area
Immunology
