Product name
Recombinant Mouse IGF-1 protein
Code
CD00406
Biological activity
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using serum free human MCF-7 cells is less than 2 ng/ml, corresponding to a specific activity of > 5.0 × 105 IU/mg.
Endotoxin
Less than 0.1 EU/μg of rMuIGF-1 as determined by LAL method.
Synonyms
IGF-1, C730016P09Rik, Igf, Igf-, Igf1, Igf-I,Mouse
Sequence
GPETLCGAEL VDALQFVCGP RGFYFNKPTG YGSSIRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPTKAA
Uniprot Accession
Molecular Weight
Approximately 7.7 kDa, a single non-glycosylated polypeptide chain containing 70 amino acids.
Formulation
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH7.4.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 100mM HAc to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Background
The insulin-like growth factors (IGFs) belonged to the insulin gene family, are mitogenic polypeptide growth factors that stimulate the proliferation and survival of various cell types including muscle, bone, and cartilage tissue in vitro. The IGFs are similar by structure and function to insulin, but have a much higher growth-promoting activity than insulin. IGF-1 is produced primarily by the liver as an endocrine hormone as well as in target tissues in a paracrine/autocrine fashion. The production of IGF-1 is stimulated by growth hormone (GH) and can be retarded by undernutrition, growth hormone insensitivity, lack of growth hormone receptors, or failures of the downstream signaling pathway post GH receptor including SHP2 and STAT5B. Recombinant Mouse IGF-1 are globular proteins containing 70 amino acids and 3 intra-molecular disulfide bonds. Mature Mouse IGF-1 shares 94 % and 98 % a.a. sequence identity with human and rat IGF-1, respectively.
Storage
-20ºC
Research area
Others
