Product name
Recombinant Mouse IFN- Lambda2/IL-28A protein
Code
CD00408
Biological activity
Fully biologically active when compared to standard. The ED50 as determined by an anti-viral assay using human HepG2 cells infected with encephalomyocarditis is less than 3 ng/ml, corresponding to a specific activity of > 3.3 × 105 IU/mg.
Endotoxin
Less than 0.1 EU/μg of rMuIFN-λ2/IL-28A as determined by LAL method.
Synonyms
IFN-λ2/IL-28A, Il; Il28a; IL-28A; If1ia1; EG330496,Ifnl2,Mouse
Sequence
DPVPRATRLP VEAKDCHIAQ FKSLSPKELQ AFKKAKDAIE KRLLEKDMRC SSHLISRAWD LKQLQVQERP KALQAEVALT LKVWENMTDS ALATILGQPL HTLSHIHSQL QTCTQLQATA EPKPPSRRLS RWLHRLQEAQ SKETPGCLED SVTSNLFRLL TRDLKCVASG DQCV
Uniprot Accession
Molecular Weight
Approximately 19.7 kDa, a single non-glycosylated polypeptide chain containing 174 amino acids.
Formulation
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4, with 5 % trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Background
Mouse IFN-lambda 2 (IL-28A), IFN-lambda 3 (IL-28B) are class II cytokine receptor ligands that are distantly related to members of the IL-10 family (11 - 13% amino acid sequence identity) and type I IFN family (15 - 19% a.a. sequence identity). These cytokines exert bioactivities that overlap those of type I IFNs, including antiviral activity and up-regulation of MHC class I antigen expression. The proteins signal through the same heterodimeric receptor complex that is composed of the IL-10 receptor beta (IL-10 R beta) and a novel IL-28 receptor alpha (IL-28 R alpha, also known as IFN-lambda R1). Mouse IL-28A shares 64%, 65%, and 56% a.a. identity with human IL-28A, IL-28B, and IL-29, respectively.
Storage
-20ºC
Research area
Immunology
