Product name
Recombinant Mouse FGF-21 protein
Code
CD00455
Biological activity
Fully biologically active when compared to standard. The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 μg/ml, corresponding to a specific activity of > 2.0 × 103 IU/mg in the presence of 5 μg/ml of rMuKlotho-β and 10 μg/ml of heparin.
Endotoxin
Less than 1 EU/μg of rMuFGF-21 as determined by LAL method.
Synonyms
FGF-21, Fgf8c,FGF21,Mouse
Sequence
AYPIPDSSPL LQFGGQVRQR YLYTDDDQDT EAHLEIREDG TVVGAAHRSP ESLLELKALK PGVIQILGVK ASRFLCQQPD GALYGSPHFD PEACSFRELL LEDGYNVYQS EAHGLPLRLP QKDSPNQDAT SWGPVRFLPM PGLLHEPQDQ AGFLPPEPPD VGSSDPLSMV EPLQGRSPSY AS
Uniprot Accession
Molecular Weight
Approximately 19.9 kDa, a single non-glycosylated polypeptide chain containing 182 amino acids.
Formulation
Lyophilized from a 0.2 μm filtered concentrated solution in 3 × PBS, pH 7.4.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Background
Fibroblast growth factor-21 (FGF-21) belongs to the large FGF family and it is specifically induced by HMGCS2 activity. In mice, brown adipose tissue becomes a source of systemic FGF21 after cold exposure. FGF-21 stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression) and the activity depends on the presence of KLB. FGF-21, in the presence of betaKlotho as a protein cofactor, signals through the FGFR 1c and 4 receptors. Recombinant Mouse FGF-21 contains 182 amino acids residues and show limited binding to heparin. In addition, Mouse FGF-21 respectively shows 81 % and 92 % a.a. identity to human and rat FGF-21, and it show activity on human and rat cells.
Storage
-20ºC
Research area
Immunology
