Product name
Recombinant Mouse Bcl-xL protein
Code
CD00616
Biological activity
Test in Process.
Endotoxin
Less than 0.1 EU/μg of rMuBcl-xL as determined by LAL method.
Synonyms
Bcl2l1, Bcl, Bcl(X, Bcl(X)L, Bcl-XL, Bcl2l, BclX, bcl-, bcl-x, bcl2-L-1, Mouse
Sequence
SQSNRELVVD FLSYKLSQKG YSWSQFSDVE ENRTEAPEET EAERETPSAI NGNPSWHLAD SPAVNGATGH SSSLDAREVI PMAAVKQALR EAGDEFELRY RRAFSDLTSQ LHITPGTAYQ SFEQVVNELF RDGVNWGRIV AFFSFGGALC VESVDKEMQV LVSRIASWMA TYLNDHLEPW IQENGGWDTF VDLYGNNAAA ESRKGQERFN R
Uniprot Accession
Molecular Weight
Approximately 23.7 kDa, a single non-glycosylated polypeptide chain containing 211 amino acids.
Formulation
Lyophilized from a 0.2 μm filtered concentrated solution in 2 × PBS, pH 7.4, 5 % Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Background
Bcl-xL is a transmembrane protein located in the mitochondrial membranes of cells that are long-lived and postmitotic, such as adult brain cells. It plays a role in the signal transduction pathway of the FAS-Ligand. Bcl-xL is an anti-apoptotic protein which is a member of the Bcl-2 family which are able to form heterodimers, and this is an significant event in the regulation of apoptosis. It is involved in the survival of cancer cells. Bcl-xL is the leading monitor of apoptosis/active cell suicide. It has cell death repressor activity and therefore acts as a survival protein. The Mouse Bcl-xL shares 97 % and 99 % a.a. sequence identity with human and rat Bcl-xL, respectively.
Storage
-20ºC
Research area
Cell Biology
