Product name
Recombinant Human UBE2R1 protein (His Tag)
Code
CD00724
Biological activity
Data is not available.
Endotoxin
Less than 0.1 EU/μg of rHuUBE2R1 as determined by LAL method.
Synonyms
CDC34,UBE2R1, E2-CDC34, UBC3, UBCH3, Human
Sequence
MHHHHHHAMG ILMARPLVPS SQKALLLELK GLQEEPVEGF RVTLVDEGDL YNWEVAIFGP PNTYYEGGYF KARLKFPIDY PYSPPAFRFL TKMWHPNIYE TGDVCISILH PPVDDPQSGE LPSERWNPTQ NVRTILLSVI SLLNEPNTFS PANVDASVMY RKWKESKGKD REYTDIIRKQ VLGTKVDAER DGVKVPTTLA EYCVKTKAPA PDEGSDLFYD DYYEDGEVEE EA
Uniprot Accession
Molecular Weight
Approximately 26.7 kDa, a single non-glycosylated polypeptide chain containing 236 amino acids.
Formulation
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, 1mM DTT, pH 7.5.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Background
Ubiquitin-conjugating enzyme E2 R1 belongs to the ubiquitin-conjugating enzyme family and is encoded by the UBE2R1 gene in humans. The ubiquitin-conjugating enzymes, also known as E2 enzymes and more rarely as ubiquitin-carrier enzymes, take part in the second step in the ubiquitination reaction. In this reaction, E1 activates the ubiquitin by covalently attaching the molecule to its active site cysteine residue. The activated ubiquitin is then transferred to an E2 cysteine and then the E2 molecule binds E3 via a structurally conserved binding region. UBE2R1 is important in the control of cell cycle and DNA replication. In vitro it catalyzes Lys-48-linked polyubiquitination. Additionally, the protein cooperates with the UBCH5C and the SCF ligase complex for the polyubiquitination of NFKBIA leading to its subsequent proteasomal degradation.
Storage
-20ºC
Research area
Others
