Product name
Recombinant Human UBE2D3 protein (His Tag)
Code
CD00728
Biological activity
Data is not available.
Endotoxin
Less than 1 EU/μg of rHuUBE2D3/UBC5C, His as determined by LAL method.
Synonyms
UBE2D3,E2(17)KB3, UBC4/5, UBCH5C, Human
Sequence
MHHHHHHAMA LKRINKELSD LARDPPAQCS AGPVGDDMFH WQATIMGPND SPYQGGVFFL TIHFPTDYPF KPPKVAFTTR IYHPNINSNG SICLDILRSQ WSPALTISKV LLSICSLLCD PNPDDPLVPE IARIYKTDRD KYNRISREWT QKYAM
Uniprot Accession
Molecular Weight
Approximately 17.7 kDa, a single non-glycosylated polypeptide chain containing 147 amino acids (a.a.) of human UBE2D3/UBC5C and 8 a.a. vector sequence including 6 × His tag at N-terminus.
Formulation
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, 1mM DTT, pH 7.5.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Background
Ubiquitin-conjugating enzyme E2 D3 belongs to the ubiquitin-conjugating enzyme family and is encoded by the UBE2D3 gene in humans. The ubiquitin-conjugating enzymes, also known as E2 enzymes and more rarely as ubiquitin-carrier enzymes, take part in the second step in the ubiquitination reaction. In this reaction, E1 activates the ubiquitin by covalently attaching the molecule to its active site cysteine residue. The activated ubiquitin is then transferred to an E2 cysteine and then the E2 molecule binds E3 via a structurally conserved binding region. The ubiquitination reaction can modify proteins and regulate protein degradation. The UBE2D3 is a human homolog of the yeast UBC4/5 family and play many important regulatory roles in inflammation and cancer. It mediates the degradation of a myriad of short-lived regulatory proteins (such as p53 in the presence of E6/E6-AP or MDM2, c-Fos, IκBα, p105) and abnormal proteins and has 88% and 89% sequence identity with UbcH5a and UbcH5b respectively.
Storage
-20ºC
Research area
Others
