Product name
Recombinant Human SDC-4 protein (His Tag)
Code
CD00954
Biological activity
Fully biologically active when compared to standard. The specific activity is determined by binding ability in a functional ELISA. Immobilized rHuSDC-4 at 500 ng/ml (100 μl/well) can bind rHubFGF with a linear range of 0.1-10 ng/ml.
Endotoxin
Less than 0.1 EU/μg of rHuSYND4 as determined by LAL method.
Synonyms
SDC-4, SYND4, Human
Sequence
MASSIRETEV IDPQDLLEGR YFSGALPDDE DVVGPGQESD DFELSGSGDL DDLEDSMIGP EVVHPLVPLD NHIPERAGSG SQVPTEPKKL EKNEVIPKRI SPVEESEDVS NKVSMSSTVQ GSNIFERTEV LALEHHHHHH
Uniprot Accession
Molecular Weight
Approximately 13.9 kDa, a single non-glycosylated polypeptide chain containing 127 amino acids. But it migrates with an apparent molecular mass of 22 kDa in SDS-PAGE.
Formulation
Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Background
Syndecan-4 (SDC-4) encoded by the SDC-4 gene in humans, has a molecular weight of ~20 kDa. It is one of the four vertebrate syndecans which belong to the syndecan family of Type 1 transmembrane proteins and capable of carrying heparin sulfate (HS) and chondroitin sulfate glycosaminoglycans. Syndecans are the best-characterized plasma membrane proteoglycans with two conserved cytoplasmic domains and divergent extracellular portions, except for HS attachment sites. SDC-4 is the most similar to SDC-2, but is more universally expressed and is found in virtually every cell type. Expression can be upregulated by TGFβ2 and in response to mechanical stress in smooth muscle, wound healing, arterial injury or acute myocardial infarction, probably in response to at least one inflammatory mediator. SDC-4 has more widespread distribution than other syndecans and it is the only syndecan that has been found consistently in focal adhesions. Human SDC-4 ECD shares approximately 79 %, 78 % and 81% a.a. identity with mouse, rat and porcine SDC-4 ECD, respectively.
Storage
-20ºC
Research area
Others
