Product name
Recombinant Human MCP-3/CCL7 protein
Code
CD01314
Biological activity
Fully biologically active when compared to standard. The biologically active determined by a chemotaxis bioassay using human monocytes is in a concentration range of 10-100 ng/ml.
Endotoxin
Less than 1 EU/μg of rHuMCP-3/CCL7 as determined by LAL method.
Synonyms
MCP-3/CCL7, A-152E5.3, ABCD-2, SCYA17, TARC, Human
Sequence
QPVGINTSTT CCYRFINKKI PKQRLESYRR TTSSHCPREA VIFKTKLDKE ICADPTQKWV QDFMKHLDKK TQTPKL
Uniprot Accession
Molecular Weight
Approximately 9.0 kDa, a single, non-glycosylated polypeptide chain containing 76 amino acids.
Formulation
Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 C. Further dilutions should be made in appropriate buffered solutions.
Background
Human CCL7, also known as monocyte chemotactic protein 3 (MCP-3), is belonging to the CC chemokine family. MCP-3 has two homogeneous MCP-1 (CCL2) and MCP-2 (CCL8). These three MCPs were found by IL-1-beta triggered human MG-63 osteosarcoma cells. CCL7 shares 71 % and 58 % a.a. sequence identity with MCP-1 and MCP-2, respectively. CCL7 has chemotactic function for monocytes and eosinophils, but not for neutrophils. In addition, it can also chemoattract activated NK cells.
Storage
-20ºC
Research area
Immunology
