Product name
Recombinant Human Maspin protein
Code
CD01329
Biological activity
Data is not available.
Endotoxin
Less than 1 EU/μg of rHuMaspin as determined by LAL method.
Synonyms
SERPINB5,PI5,Maspin, Human
Sequence
MDALQLANSA FAVDLFKQLC EKEPLGNVLF SPICLSTSLS LAQVGAKGDT ANEIGQVLHF ENVKDIPFGF QTVTSDVNKL SSFYSLKLIK RLYVDKSLNL STEFISSTKR PYAKELETVD FKDKLEETKG QINNSIKDLT DGHFENILAD NSVNDQTKIL VVNAAYFVGK WMKKFPESET KECPFRLNKT DTKPVQMMNM EATFCMGNID SINCKIIELP FQNKHLSMFI LL
Uniprot Accession
Molecular Weight
Approximately 42.1 kDa, a single non-glycosylated polypeptide chain containing 375 amino acid residues.
Formulation
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Background
Mammary serine protease inhibitor (Maspin), also known as serpin B5 is a non-inhibitory serpin that is encoded by the SERPINB5 gene in humans. The protein is expressed predominantly in normal mammary epithelial cells but at significantly reduced levels or absent in most breast carcinomas. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity. The maspin functions as tumor suppressor, blocking the growth, invasion, and metastatic properties of mammary tumors. This anti-tumor activity is achieved, in part, by the ability of maspin to inhibit angiogenesis and to preferentially promote apoptosis of tumor cells.
Storage
-20ºC
Research area
Others
