Product name
Recombinant Human IL-2 protein
Code
CD01529
Biological activity
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using Mouse CTLL-2 cells is less than 0.1 ng/ml, corresponding to a specific activity of > 1.0 × 107 IU/mg.
Endotoxin
Less than 1.0 EU/μg of rHuIL-2 as determined by LAL method.
Synonyms
IL-2, IL2, TCGF, lymphokine,Human
Sequence
APTSSSTKKT QLQLEHLLLD LQMILNGINN YKNPKLTRML TFKFYMPKKA TELKHLQCLE EELKPLEEVL NLAQSKNFHL RPRDLISNIN VIVLELKGSE TTFMCEYADE TATIVEFLNR WITFCQSIIS TLT
Uniprot Accession
Molecular Weight
Approximately 15.4 kDa, a single non-glycosylated polypeptide chain containing 133 amino acids.
Formulation
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 5.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled H2O to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. Do not reconstitute in cell culture media directly.
Background
IL-2 is a powerful immunoregulatory lymphokine produced by T-cells in response to antigenic or mitogenic stimulation. It is expressed by CD4+ and CD8+ T cells, γδ T cells, B cells, dendritic cells, and eosinophils. IL-2/IL-2R signaling is required for T-cell proliferation and other fundamental functions which are essential for the immune response. The receptor for IL-2 consists of three subunits (55 kDa IL-2Rα, 75 kDa IL-2Rβ, and 64 kDa common gamma chain γc/IL-2Rγ) that are present on the cell surface in varying preformed complexes. Mature human IL-2 shares 56 % and 66 % amino acid sequence identity with mouse and rat IL-2, respectively. Human and mouse IL-2 exhibit cross-species activity.
Storage
-20ºC
Research area
Immunology
