Product name
Recombinant Human IL-16 protein
Code
CD01559
Biological activity
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral T Lymphocytes is in a concentration range of 50-500 ng/ml.
Endotoxin
Less than 1EU/μg of rHuIL-16, 121 a.a. as determined by LAL method.
Synonyms
IL-16,LCF, NIL16, PRIL16, prIL-16,IL16, Human
Sequence
SAASASAASD VSVESTAEAT VCTVTLEKMS AGLGFSLEGG KGSLHGDKPL TINRIFKGAA SEQSETVQPG DEILQLGGTA MQGLTRFEAW NIIKALPDGP VTIVIRRKSL QSKETTAAGD S
Uniprot Accession
Molecular Weight
Approximately 12.4 kDa, a single non-glycosylated polypeptide chain containing 121 amino acids.
Formulation
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Background
Human Interleukin-16 (IL-16) is also called lymphocyte chemoattractant factor (LCF) and it has three isoforms which are cleaved from pre-Interleukin-16. IL-16 is mostly secreted by lymphocytes, epithelial cells, eosinophils, and CD8+ T cells. It has many functions including induction of the IL-2Rα on T cells, suppression of human immunodeficiency virus (HIV) replication, inhibition of T-cell antigen receptor/CD3 mediated T-cell stimulation in mixed lymphocyte reactions and so on. It signals through CD4 receptor. Furthermore, recombinant human IL-16 (121 a.a.) contains 121 amino acid residues and it shares 85 % a.a. sequence identity with Mouse IL-16.
Storage
-20ºC
Research area
Immunology
