Product name
Recombinant Human IHH C28II protein
Code
CD01574
Biological activity
Fully biologically active when compared to standard. The ED50 as determined by its ability to induce alkaline phosphatase production by C3H10T1/2(CCL-226) cells is 3.0-10 μg/ml.
Endotoxin
Less than 1 EU/μg of rHuIHH C28II as determined by LAL method.
Synonyms
IHH C28II,BDA1, HHG2, Human
Sequence
IIGPGRVVGS RRRPPRKLVP LAYKQFSPNV PEKTLGASGR YEGKIARSSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDRLNSLAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRAVDITTS DRDRNKYGLL ARLAVEAGFD WVYYESKAHV HCSVKSEHSA AAKTGG
Uniprot Accession
Molecular Weight
Approximately 19.8 kDa, a single non-glycosylated polypeptide chain containing 176 amino acids.
Formulation
Lyophilized from a 0.2 μm filtered concentrated solution in 1 × PBS, pH 7.4.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Background
IHH, encoded by the IHH gene in humans, belongs to the mammalian hedgehog family. It is expressed in adult kidney and liver. The function of IHH is involved in chondrocyte differentiation, proliferation and maturation especially during endochondral ossification. It regulates its effects by feedback control of parathyroid hormone-related peptide (PTHrP). IHH is also involved in yolk sac vasculogenesis, playing an important role in differentiation of epiblast cells into endothelial and red blood cells. Human IHH shares 100 % amino acid sequence identity with Mouse.
Storage
-20ºC
Research area
Others
