Recombinant Equine Interferon-gamma protein
Fully biologically active when compared to standard. The ED50 as determined by an anti - viral assay using human HeLa cells infected w ith encephalomyocarditis (EMC) virus is less than 10.0 ng/ml, corresponding to a specific activity of > 1.0 × 105 IU/mg.
Less than 0. 1 EU/ μ g of rEqIFN -γ as determined by LAL method.
QAAFFKEIEN LKEYFNASNP DVGDGGPLFL DILKNWKEDS DKKIIQSQIV SFYFKLFENL KDNQVIQKSM DTIKEDLFVK FFNSSTSKLE DFQK LIQIPV NDLKVQRKAI SELIKVMNDL SPKANLRKRK RSQNPFRGRR ALQ
Approximately 16.7 kDa, a single non-glycosylated polypeptide chain containing 143 amino acids.
Lyophilized from a 0.2 μ m filtered concen trated solution in 2 × PBS, pH 7.4, with 5 % trehalose .
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Interferon- gamma (IFN-γ), also known as Type II interferon or immune interferon, is a cytokine produced primarily by T -lymphocytes and natural killer cells. The protein shares no significant homology with IFN-β or the various IFN- α family proteins. Mature IFN-γ exists as noncovalently-linked homodimers. IFN-γ was originally characterized based on its antiviral activities. The protein also exerts antiproliferative, immunoregulatory and proinflammatory activities and is thus important in host defense mechanis ms. IFN-γ induces the production of cytokines, upregulates the expression of class I and II MHC antigens, Fc receptor and leukocyte adhesion molecules. It modulates macrophage effector functions, influences isotype switching and potentiates the secretion of immunoglobulins by B cells. Additionally, IFN-γ augments TH1 cell expansion and may be required for TH1 cell differentiation. Equine IFN-γ shares 73%~ 82% amino acid sequence identity with bovine, canine, feline, and porcine IFN-γ and 42 %~64 % with cotton rat, human, Mouse, rat, and rhesus macaque IFN-γ .