AFP Alpha 1 Fetoprotein Monoclonal Antibody(17C5)

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Home > Product > Antibody > AFP Alpha 1 Fetoprotein Monoclonal Antibody(17C5)

AFP Alpha 1 Fetoprotein Monoclonal Antibody(17C5)

Product Summary

Size: 20ul,50ul,100ul

Clonality: Monoclonal

Host: Mouse

Reactivity:Human

Applications: WB, IHC-p, IF, ICC

Product Summary

Size: 20ul,50ul,100ul

Clonality: Monoclonal

Host: Mouse

Reactivity: Human

Applications: WB, IHC-p, IF, ICC

Product overview

Full product name

Alpha-fetoprotein

Code

BT-MCA0003

Host

Mouse

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Monoclonal

Immunogen

Synthetic Peptide of AFP alpha 1 Fetoprotein

Purification

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Concentration

1 mg/ml

Formulation

PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

Storage

-20°C for one year

Background

This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly.

Uniprot accession

Human: P02771; Mouse: P02772; Rat: P02773;

Molecular weight

N/A

Gene ID

Human: 174; Mouse: 11576

Synonyms

AFP; HPAFP; Alpha-fetoprotein; Alpha-1-fetoprotein; Alpha-fetoglobulin

Research area

Others

Target protein

AFP

Recommended dilution

Application	Dilution
IF	1:200
IHC	1:50-300
WB	1:2000

Images

Immunohistochemical analysis of paraffin-embedded Human-breast-cancer tissue. 1.AFP alpha 1 Fetoprotein Monoclonal antibody(17C5) was diluted at 1:200(4°C,overnight). 2.Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3.Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.

Immunofluorescence analysis of Human-breast-cancer tissue. 1.AFP alpha 1 Fetoprotein Monoclonal antibody(17C5)(red) was diluted at 1:200(4°C,overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Western blot analysis of HepG2. diluted at 1:2.000.

Immunohistochemical analysis of paraffin-embedded Human gallbladder.1.Antibody was diluted at 1:100(4°C overnight).2.High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature, 30min).

Immunohistochemical analysis of paraffin-embedded Human gallbladder.1.Antibody was diluted at 1:100(4°C overnight). 2.High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature, 30min).

Immunohistochemical analysis of paraffin-embedded Human Prostate. 1.Antibody was diluted at 1:100(4°C overnight). 2.High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature, 30min).

Immunohistochemical analysis of paraffin-embedded Human Prostate.1.Antibody was diluted at 1:100(4°C overnight). 2.High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature, 30min).

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

20ul

$108.00
50ul

$180.00
100ul

$275.00

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Lead time: Within one week

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