Tel:86 021 3100 7137

Publish and get free product : share with us your publications using BT LAB product and get free products.
Home > Product > Antibody > GAPDH Monoclonal Antibody(2B8)

GAPDH Monoclonal Antibody(2B8)

GAPDH Monoclonal Antibody(2B8)

Product Summary

Size: 20ul,50ul,100ul

Clonality: Monoclonal

Host: Mouse

Reactivity:Human, Rat, Mouse, Monkey, Dog, Chicken, Hamster, Rabbit, Pig, sheep, Insect, Yeast

Applications: WB, IF, ICC, IHC-p

Product Summary

Size: 20ul,50ul,100ul

Clonality: Monoclonal

Host: Mouse

Reactivity: Human,Rat,Mouse,Monkey,Dog,Chicken,Hamster,Rabbit,Pig,sheep,Insect,Yeast

Applications: WB, IF, ICC, IHC-p

Product overview

Full product name

Glyceraldehyde-3-phosphate dehydrogenase

Code

BT-MCA0039

Host

Mouse

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Monoclonal

Immunogen

Synthetic Peptide of GAPDH

Purification

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Concentration

1 mg/ml

Formulation

PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

Storage

-20°C for one year

Background

This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferri

Uniprot accession

Molecular weight

N/A

Gene ID

Human: 2597; Mouse: 100042025; Rat: 24383

Synonyms

GAPDH; GAPD; CDABP0047; OK; SW-cl.12; Glyceraldehyde-3-phosphate dehydrogenase; GAPDH; Peptidyl-cysteine S-nitrosylase GAPDH

Research area

Cancer; Signal transduction; Neuroscience; Metabolism

Target protein

GAPDH

Recommended dilution

Application Dilution
IF 1:200
IHC 1:200-300
WB 1:5000-20000

Images

Immunofluorescence analysis of Hela cell. Cyclin D1 Polyclonal Antibody(red) was diluted at 1:200(4°C overnight). GAPDH Monoclonal antibody(2B8)(green) was diluted at 1:200(4°C overnight).
Western blot analysis of 1 HEK293 2 SW480 3 HEPG2 4 MCF-7 5 mouse brain 6 Rat brain 7 Hela 8 A549 lysates, primary antibody was diluted at 1:5000, 4°C overnight, secondary antibody was diluted at 1:10000, 37°C 1hour.
Immunohistochemical analysis of paraffin-embedded Human-colon tissue. 1.GAPDH Monoclonal antibody(2B8) was diluted at 1:200(4°C,overnight). 2.Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3.Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1.GAPDH Monoclonal antibody(2B8) was diluted at 1:200(4°C,overnight). 2.Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3.Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Mouse-heart tissue. 1.GAPDH Monoclonal antibody(2B8) was diluted at 1:200(4°C,overnight). 2.Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3.Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Immunofluorescence analysis of Human-colon tissue. 1.GAPDH Monoclonal antibody(2B8)(red) was diluted at 1:200(4°C,overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunofluorescence analysis of Mouse-liver tissue. 1.GAPDH Monoclonal antibody(2B8)(red) was diluted at 1:200(4°C,overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Western blot analysis of Hela (1) Rat brain (2) Rabbit Muscle (3) Sheep Muscle (4) and Mouse brain (5) diluted at 1:10000.

Protocols

References

Customer reviews and Q&As

No reviews
No reviews

Product size

Price

  • 20ul

    $108.00

  • 50ul

    $180.00

  • 100ul

    $275.00

Add to Cart

Lead time: Within one week

We are continuously updating our Antibody catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

Newsletter

Sign up

Newsletter

Sign up