GFAP Monoclonal Antibody(5C8)

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Home > Product > Antibody > GFAP Monoclonal Antibody(5C8)

GFAP Monoclonal Antibody(5C8)

Product Summary

Size: 20ul,50ul,100ul

Clonality: Monoclonal

Host: Mouse

Reactivity:Human, Rat, Mouse

Applications: WB, IHC-p, IF, ICC

Product Summary

Size: 20ul,50ul,100ul

Clonality: Monoclonal

Host: Mouse

Reactivity: Human,Rat,Mouse

Applications: WB, IHC-p, IF, ICC

Product overview

Full product name

Glial fibrillary acidic protein

Code

BT-MCA0040

Host

Mouse

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Monoclonal

Immunogen

Synthetic Peptide of GFAP

Purification

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Concentration

1 mg/ml

Formulation

PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

Storage

-20°C for one year

Background

This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Uniprot accession

Human: P14136; Mouse: P03995; Rat: P47819;

Molecular weight

49880

Gene ID

Human: 2670; Mouse: 14580; Rat: 24387

Synonyms

GFAP; Glial fibrillary acidic protein; GFAP

Research area

Tags & cell markers; Signal transduction; Neuroscience; Stem cells

Target protein

GFAP

Recommended dilution

Application	Dilution
IF	1:200
IHC	1:50-300
WB	1:2000-5000

Images

Immunofluorescence analysis of Hela cell. AR Polyclonal Antibody(red) was diluted at 1:200(4°C overnight). GFAP Monoclonal antibody(5C8)(green) was diluted at 1:200(4°C overnight).

Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1.GFAP Monoclonal antibody(5C8) was diluted at 1:200(4°C,overnight). 2.Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3.Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Rat-heart tissue. 1.GFAP Monoclonal antibody(5C8) was diluted at 1:200(4°C,overnight). 2.Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3.Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Mouse-kidney tissue. 1.GFAP Monoclonal antibody(5C8) was diluted at 1:200(4°C,overnight). 2.Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3.Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.

Immunofluorescence analysis of Mouse-brain tissue. 1.GFAP Monoclonal antibody(5C8)(red) was diluted at 1:200(4°C,overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunofluorescence analysis of Rat-brain tissue. 1.GFAP Monoclonal antibody(5C8)(red) was diluted at 1:200(4°C,overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Western blot analysis of Rat Brain Tissue diluted at 1:5000.

Western blot analysis of lysates from Rat Brain Tissue, HeLa, A431 and PC12 cells, (Green) primary antibody was diluted at 1:1000, 4°overnight, secondary antibody was diluted at 1:10000, 37°C 1hour. (Red) Tubulin Beta Polyclonal Antibody antibody was diluted at 1:5000 as loading control, 4°C overnight,secondary antibody was diluted at 1:10000, 37°C 1hour.

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

20ul

$108.00
50ul

$180.00
100ul

$275.00

Add to Cart

Lead time: Within one week

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