Oct1 Monoclonal Antibody(7G1)

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Home > Product > Antibody > Oct1 Monoclonal Antibody(7G1)

Oct1 Monoclonal Antibody(7G1)

Product Summary

Size: 20ul,50ul,100ul

Clonality: Monoclonal

Host: Mouse

Reactivity:Human

Applications: WB, IHC-p, IF, ICC,

Product Summary

Size: 20ul,50ul,100ul

Clonality: Monoclonal

Host: Mouse

Reactivity: Human

Applications: WB, IHC-p, IF, ICC,

Product overview

Full product name

POU domain class 2 transcription factor

Code

BT-MCA0066

Host

Mouse

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Monoclonal

Immunogen

Synthetic Peptide of 42644

Purification

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Concentration

1 mg/ml

Formulation

PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

Storage

-20°C for one year

Background

The OCT1 transcription factor was among the first identified members of the POU transcription factor family (summarized by Sturm et al., 1993 [PubMed 8314572]). Members of this family contain the POU domain, a 160-amino acid region necessary for DNA binding to the octameric sequence ATGCAAAT.[supplied by OMIM, Jul 2010],

Uniprot accession

Human: P14859; P09086P31503: ;

Molecular weight

76472

Gene ID

Human: 5451; 5452; Mouse: 18986; 18987; Rat: 171068

Synonyms

Research area

Epigenetics and nuclear signaling

Target protein

POU2F1

Recommended dilution

Application	Dilution
IF	1:200
IHC	1:50-300
WB	1:500-2000

Images

Immunohistochemical analysis of paraffin-embedded Human-breast-cancer tissue. 1.Oct1 Monoclonal antibody(7G1) was diluted at 1:200(4°C,overnight). 2.Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3.Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.

Immunofluorescence analysis of Human-breast-cancer tissue.1.Oct1 Monoclonal antibody(7G1)(red) was diluted at 1:200(4°C,overnight). 2.Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3.Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Western blot analysis of 1) Hela, 2) Jurkat, 3) HepG2. diluted at 1:2000

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

20ul

$108.00
50ul

$180.00
100ul

$275.00

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Lead time: Within one week

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