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Home > Product > Antibody > PCNA Monoclonal Antibody(12D10)

PCNA Monoclonal Antibody(12D10)

PCNA Monoclonal Antibody(12D10)

Product Summary

Size: 20ul,50ul,100ul

Clonality: Monoclonal

Host: Mouse

Reactivity:Human, Rat, Mouse

Applications: WB, IF, ICC, IHC-p

Product Summary

Size: 20ul,50ul,100ul

Clonality: Monoclonal

Host: Mouse

Reactivity: Human,Rat,Mouse

Applications: WB, IF, ICC, IHC-p

Product overview

Full product name

Proliferating cell nuclear antigen

Code

BT-MCA0068

Host

Mouse

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Monoclonal

Immunogen

Synthetic Peptide of PCNA

Purification

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Concentration

1 mg/ml

Formulation

PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

Storage

-20°C for one year

Background

The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.

Uniprot accession

Molecular weight

28769

Gene ID

Human: 5111; Mouse: 18538; Rat: 25737

Synonyms

PCNA; Proliferating cell nuclear antigen; PCNA; Cyclin

Research area

Cancer; Cell biology; Epigenetics and nuclear signaling; Tags & cell markers

Target protein

PCNA

Recommended dilution

Application Dilution
IF 1:200
IHC 1:200
WB 1:5000

Images

Immunofluorescence analysis of Hela cell. 14-3-3 Theta/τ (phospho Ser232) Polyclonal Antibody(green) was diluted at 1:200(4°C overnight). (red) was diluted at 1:200(4°C overnight).
Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1.PCNA Monoclonal antibody(12D10) was diluted at 1:200(4°C,overnight). 2.Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3.Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-testis tissue. 1.PCNA Monoclonal antibody(12D10) was diluted at 1:200(4°C,overnight). 2.Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3.Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Mouse-liver tissue. 1.PCNA Monoclonal antibody(12D10) was diluted at 1:200(4°C,overnight). 2.Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3.Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Immunofluorescence analysis of Human-lung-cancer tissue. 1.PCNA Monoclonal antibody(12D10)(red) was diluted at 1:200(4°C,overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunofluorescence analysis of Rat-testis tissue. 1.PCNA Monoclonal antibody(12D10)(red) was diluted at 1:200(4°C,overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Western blot analysis of Hela (1) Rat brain (2) NIH 3T3 (3) 293T (4) diluted at 1:5000.

Protocols

References

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Product size

Price

  • 20ul

    $108.00

  • 50ul

    $180.00

  • 100ul

    $275.00

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Lead time: Within one week

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