XRCC4 Monoclonal Antibody(5C10)

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Home > Product > Antibody > XRCC4 Monoclonal Antibody(5C10)

XRCC4 Monoclonal Antibody(5C10)

Product Summary

Size: 20ul,50ul,100ul

Clonality: Monoclonal

Host: Mouse

Reactivity:Human

Applications: WB, IHC-p, IF, ICC, IP

Product Summary

Size: 20ul,50ul,100ul

Clonality: Monoclonal

Host: Mouse

Reactivity: Human

Applications: WB, IHC-p, IF, ICC, IP

Product overview

Full product name

DNA repair protein XRCC4

Code

BT-MCA0081

Host

Mouse

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Monoclonal

Immunogen

Synthetic Peptide of XRCC4

Purification

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Concentration

1 mg/ml

Formulation

PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

Storage

-20°C for one year

Background

The protein encoded by this gene functions together with DNA ligase IV and the DNA-dependent protein kinase in the repair of DNA double-strand breaks. This protein plays a role in both non-homologous end joining and the completion of V(D)J recombination. Mutations in this gene can cause short stature, microcephaly, and endocrine dysfunction (SSMED). Alternative splicing generates several transcript variants.

Uniprot accession

Human: Q13426; Mouse: Q924T3;

Molecular weight

38287

Gene ID

Human: 7518; Mouse: 108138

Synonyms

XRCC4; DNA repair protein XRCC4; X-ray repair cross-complementing protein 4

Research area

Epigenetics and nuclear signaling

Target protein

XRCC4

Recommended dilution

Application	Dilution
IF	1:200
IHC	1:50-300
IP	1:200
WB	1:2000

Images

Immunofluorescence analysis of Hela cell. Bak Polyclonal Antibody(green) was diluted at 1:200(4°C overnight). (red) was diluted at 1:200(4°C overnight).

Immunohistochemical analysis of paraffin-embedded Human-breast-cancer tissue. 1.XRCC4 Monoclonal antibody(5C10) was diluted at 1:200(4°C,overnight). 2.Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3.Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.

Immunofluorescence analysis of Human-liver-cancer tissue. 1.XRCC4 Monoclonal antibody(5C10)(red) was diluted at 1:200(4°C,overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Western blot analysis of 1) Hela, 2) 293T diluted at 1:3000.

Protocols

> Protocol Booklet

References

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Datasheet

Product size

Price

20ul

$108.00
50ul

$180.00
100ul

$275.00

Add to Cart

Lead time: Within one week

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