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Home > Product > Antibody > Phospho-Akt (S473) mouse Monoclonal Antibody(6F8)

Phospho-Akt (S473) mouse Monoclonal Antibody(6F8)

Phospho-Akt (S473) mouse Monoclonal Antibody(6F8)

Product Summary

Size: 20ul,50ul,100ul

Clonality: Monoclonal

Host: Mouse

Reactivity:Human

Applications: WB, IHC-p, IF

Product Summary

Size: 20ul,50ul,100ul

Clonality: Monoclonal

Host: Mouse

Reactivity: Human

Applications: WB, IHC-p, IF

Product overview

Full product name

AKT1

Code

BT-MCA1022

Host

Mouse

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Monoclonal

Immunogen

Synthetic Peptide of Phospho-Akt (S473) at AA range of 410-490

Purification

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for one year

Background

The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene.

Uniprot accession

Molecular weight

N/A

Gene ID

Human: 207

Synonyms

AKT1

Research area

Signal transduction; Metabolism; Cancer; Epigenetics and nuclear signaling

Target protein

AKT1

Recommended dilution

Application Dilution
IHC 1:100-200
WB 1:1000-2000

Images

Immunohistochemical analysis of paraffin-embedded Human Lung Carcinoma Tissue using Phospho-Akt Ser473 Mouse Monoclonal antibody diluted at 1:200.
Immunohistochemical analysis of paraffin-embedded Human Breast Carcinoma Tissue using Phospho-Akt Ser473 Mouse Monoclonal antibody diluted at 1:200.
Western blot analysis of PC3 Cell Lysate using Phospho-Akt Ser473 Mouse Monoclonal antibody diluted at 1:1000.

Protocols

References

Customer reviews and Q&As

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Product size

Price

  • 20ul

    $108.00

  • 50ul

    $180.00

  • 100ul

    $275.00

Add to Cart

Lead time: Within one week

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