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Home > Product > Antibody > Smad2 Monoclonal Antibody

Smad2 Monoclonal Antibody

Smad2 Monoclonal Antibody

Product Summary

Size: 50ul,100ul

Clonality: Monoclonal

Host: Mouse

Reactivity:Human

Applications: WB, IHC-p, IF, ICC, FCM, ELISA

Product Summary

Size: 50ul,100ul

Clonality: Monoclonal

Host: Mouse

Reactivity: Human

Applications: WB, IHC-p, IF, ICC, FCM, ELISA

Product overview

Full product name

Mothers against decapentaplegic homolog 2

Code

BT-MCA1130

Host

Mouse

Isotype

IgG

Size

50ul,100ul

Clonality

Monoclonal

Immunogen

Purified recombinant fragment of human Smad2 expressed in E. Coli.

Purification

Affinity purification

Concentration

1 mg/ml

Formulation

Ascitic fluid containing 0.03% sodium azide,0.5% BSA, 50%glycerol.

Storage

-20°C for one year

Background

The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation

Uniprot accession

Molecular weight

N/A

Gene ID

Human: 4087

Synonyms

SMAD2; MADH2; MADR2; Mothers against decapentaplegic homolog 2; MAD homolog 2; Mothers against DPP homolog 2; JV18-1; Mad-related protein 2; hMAD-2; SMAD family member 2; SMAD 2; Smad2; hSMAD2

Research area

Signal transduction; Epigenetics and nuclear signaling; Cancer; Stem cells

Target protein

SMAD2

Recommended dilution

Application Dilution
FC 1:200 - 1:400
IF 1:200 - 1:1000
IHC 1:200 - 1:1000
WB 1:500 - 1:2000

Images

Western Blot analysis using Smad2 Monoclonal antibody against HEK293 (1) and SMAD2-hIgGFc transfected HEK293 (2) cell lysate.
Immunohistochemistry analysis of paraffin-embedded human liver cancer tissues with DAB staining using Smad2 Monoclonal antibody.
Immunofluorescence analysis of U251 cells using Smad2 Monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow cytometric analysis of NIH/3T3 cells using Smad2 Monoclonal antibody (blue) and negative control (red).

Protocols

References

Customer reviews and Q&As

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Product size

Price

  • 50ul

    $180.00

  • 100ul

    $300.00

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Lead time: Within one week

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