p38(Phospho Thr180/Y182) Polyclonal Antibody

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Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Human, Mouse, Rat, Guinea pig

Applications: IF, ICC, WB, IHC-p, ELISA

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse, Rat, Guinea pig

Applications: IF, ICC, WB, IHC-p, ELISA

Product overview

Full product name

Mitogen-activated protein kinase 14

Code

BT-AP01320

Host

Rabbit

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from human p38 MAPK around the phosphorylation site of Thr179 and Tyr181. AA range:151-200

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for 1 year

Background

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of this gene encoding d

Uniprot accession

Human: Q16539; Mouse: P47811; Rat: P70618;

Molecular weight

41293

Gene ID

Human: 1432; Mouse: 26416

Synonyms

Mitogen-activated protein kinase 14; MAPK14; CSBP; CSBP1; CSBP2; CSPB1; MXI2; SAPK2A; Mitogen-activated protein kinase 14; MAP kinase 14; MAPK 14; Cytokine suppressive anti-inflammatory drug-binding protein; CSAID-binding protein; CSBP; MAP kinase MXI2; MAX-interacting protein

Research area

Immunology; Signal transduction; Cancer; Cell biology; Epigenetics and nuclear signaling

Target protein

MAPK14

Recommended dilution

Application	Dilution
WB	1: 500 - 1: 2000
IHC-p	1: 100 - 1: 300
IF	1: 50 - 1: 200
ELISA	1: 5000

Images

Immunofluorescence analysis of Hela cell. 1,p38 (phospho Thr180/Y182) Polyclonal Antibody(Green) was diluted at 1:200(4°C overnight). 2 DAPI(blue) 10min.

Immunofluorescence analysis of mouse-liver tissue. 1,p38 (phospho Thr180/Y182) Polyclonal Antibody(Red) was diluted at 1:200(4°C overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunohistochemical analysis of paraffin-embedded Human-uterus-cancer tissue. 1,p38 (phospho Thr180/Y182) Polyclonal Antibody was diluted at 1:200(4°C overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Rat-spinal-cord tissue. 1,p38 (phospho Thr180/Y182) Polyclonal Antibody was diluted at 1:200(4°C overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Rat-spleen tissue. 1,p38 (phospho Thr180/Y182) Polyclonal Antibody was diluted at 1:200(4°C overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Mouse-liver tissue. 1,p38 (phospho Thr180/Y182) Polyclonal Antibody was diluted at 1:200(4°C overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Mouse-lung tissue. 1,p38 (phospho Thr180/Y182) Polyclonal Antibody was diluted at 1:200(4°C overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Mouse-kidney tissue. 1,p38 (phospho Thr180/Y182) Polyclonal Antibody was diluted at 1:200(4°C overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Mouse-brain tissue. 1,p38 (phospho Thr180/Y182) Polyclonal Antibody was diluted at 1:200(4°C overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Mouse-spleen tissue. 1,p38 (phospho Thr180/Y182) Polyclonal Antibody was diluted at 1:200(4°C overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Human-colon tissue. 1,p38 (phospho Thr180/Y182) Polyclonal Antibody was diluted at 1:200(4°C overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1,N-cadherin Polyclonal Antibody was diluted at 1:200(4°C overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Human-lung tissue. 1,p38 (phospho Thr180/Y182) Polyclonal Antibody was diluted at 1:200(4°C overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Human-lung-cancer tissue. 1,p38 (phospho Thr180/Y182) Polyclonal Antibody was diluted at 1:200(4°C overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Human-Appendix tissue. 1,p38 (phospho Thr180/Y182) Polyclonal Antibody was diluted at 1:200(4°C overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Rat-heart tissue. 1,p38 (phospho Thr180/Y182) Polyclonal Antibody was diluted at 1:200(4°C overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1,p38 (phospho Thr180/Y182) Polyclonal Antibody was diluted at 1:200(4°C overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1,p38 (phospho Thr180/Y182) Polyclonal Antibody was diluted at 1:200(4°C overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.

Western Blot analysis of various cells using primary antibody diluted at 1:1000(4°C overnight). Secondary antibody:Goat Anti-rabbit IgG IRDye 800( diluted at 1:5000, 25°C, 1 hour)

Western Blot analysis of various cells using Phospho-p38 (T180/Y182) Polyclonal Antibody diluted at 1:1000

Western Blot analysis of HEPG2 cells using Phospho-p38 (T180/Y182) Polyclonal Antibody diluted at 1:1000

Western blot analysis of K562 lysis using Phospho-p38 (T180/Y182) antibody. Antibody was diluted at 1:1000

Western blot analysis of lysates from A549 cells treated with etoposide 25uM 24hours, using p38 MAPK (Phospho-Thr179+Tyr181) Antibody. The lane on the right is blocked with the phospho peptide.

Protocols

> Protocol Booklet

References

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Datasheet

Product size

Price

20ul

$108.00
50ul

$180.00
100ul

$275.00

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Lead time: Within one week

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