Sox-9(Phospho Ser181) Polyclonal Antibody

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Blood coagulation
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Home > Product > Antibody > Sox-9(Phospho Ser181) Polyclonal Antibody

Sox-9(Phospho Ser181) Polyclonal Antibody

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Human, Mouse

Applications: WB, IHC-p, IF, ICC, ELISA

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, IHC-p, IF, ICC, ELISA

Product overview

Full product name

Transcription factor SOX-9

Code

BT-AP01621

Host

Rabbit

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from human SOX-9 around the phosphorylation site of Ser181. AA range:147-196

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for 1 year

Background

The protein encoded by this gene recognizes the sequence CCTTGAG along with other members of the HMG-box class DNA-binding proteins. It acts during chondrocyte differentiation and| with steroidogenic factor 1| regulates transcription of the anti-Muellerian hormone (AMH) gene. Deficiencies lead to the skeletal malformation syndrome campomelic dysplasia| frequently with sex reversal.

Uniprot accession

Human: P48436; Mouse: Q04887;

Molecular weight

56137

Gene ID

Human: 6662; Mouse: 20682

Synonyms

Transcription factor SOX-9; SOX9; Transcription factor SOX-9

Research area

Epigenetics and nuclear signaling; Stem cells; Developmental biology; Neuroscience

Target protein

SOX9

Recommended dilution

Application	Dilution
WB	1: 500 - 1: 2000
IHC-p	1: 100 - 1: 300
IF	1: 200 - 1: 1000
ICC	1: 200 - 1: 1000
ELISA	1: 5000

Images

Immunofluorescence analysis of A549 cells, using SOX-9 (Phospho-Ser181) Antibody. The picture on the right is blocked with the phospho peptide.

Immunohistochemistry analysis of paraffin-embedded human brain, using SOX-9 (Phospho-Ser181) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of Sox-9 (phospho Ser181) Polyclonal Antibody, using Hela, HepG2 cell treated or untreated with UV, 4°C overnight, secondary antibody was diluted at 1:10000, 37°C 1hour.

Western blot analysis of lysates from 293 cells treated with PBS 60', using SOX-9 (Phospho-Ser181) Antibody. The lane on the right is blocked with the phospho peptide.

Protocols

> Protocol Booklet

References

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Datasheet

Product size

Price

20ul

$108.00
50ul

$180.00
100ul

$275.00

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Lead time: Within one week

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