MMP1

BT-AP03630

Rabbit

IgG

100ul,50ul,20ul

Polyclonal

Synthesized peptide derived from human MMP1 (Cleaved-Ile271)

The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

1 mg/ml

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

-20°C for 1 year

Catalytic activity:Cleavage of the triple helix of collagen at about three-quarters of the length of the molecule from the N-terminus| at 775-Gly-|-Ile-776 in the alpha-1(I) chain. Cleaves synthetic substrates and alpha-macroglobulins at bonds where P1' is a hydrophobic residue.|cofactor:Binds 2 zinc ions per subunit.|cofactor:Binds 4 calcium ions per subunit.|The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion| thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.|There are two distinct domains in this protein; the catalytic N-terminal| and the C-terminal which is involved in substrate specificity and in binding TIMP (tissue inhibitor of metalloproteinases).|enzyme regulation:Can be activated without removal of the activation peptide.|Cleaves collagens of types I| II| and III at one site in the helical domain. Also cleaves collagens of types VII and X. In case of HIV infection| interacts and cleaves the secreted viral Tat protein| leading to a decrease in neuronal Tat's mediated neurotoxicity.|online information:Collagenase entry|PTM:Undergoes autolytic cleavage to two major forms (22 kDa and 27 kDa). A minor form (25 kDa) is the glycosylated form of the 22 kDa form. The 27 kDa form has no activity while the 22/25 kDa form can act as activator for collagenase.|Belongs to the peptidase M10A family.|Contains 4 hemopexin-like domains.|subunit:Interacts with HIV-1 Tat.|

Human: P03956;

51590

Human: 4312

MMP1 ;Cleaved-Ile271; Interstitial collagenase; EC 3.4.24.7; Fibroblast collagenase; Matrix metalloproteinase-1; MMP-1; 22 kDa interstitial collagenase; 27 kDa interstitial collagenase;

Cancer; Cardiovascular; Cell biology; Signal transduction

MMP1