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Home > Product > Antibody > Cdk1/2/3(Phospho Thr14) Polyclonal Antibody

Cdk1/2/3(Phospho Thr14) Polyclonal Antibody

Cdk1/2/3(Phospho Thr14) Polyclonal Antibody

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Human, Mouse, Rat, Monkey

Applications: WB, IF, ICC, ELISA

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse, Rat, Monkey

Applications: WB, IF, ICC, ELISA

Product overview

Full product name

Cyclin-dependent kinase 1/2/3

Code

BT-AP14847

Host

Rabbit

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from human CDK1/CDC2 around the phosphorylation site of Thr14. AA range:1-50

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for 1 year

Background

The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Uniprot accession

Molecular weight

33930

Gene ID

Human: 1017; Mouse: 12534/12566; Rat: 54237

Synonyms

Cyclin-dependent kinase 1/2/3; CDK1; CDC2; CDC28A; CDKN1; P34CDC2; Cyclin-dependent kinase 1; CDK1; Cell division control protein 2 homolog; Cell division protein kinase 1; p34 protein kinase; CDK2; CDKN2; Cyclin-dependent kinase 2; Cell division protein kinase 2; p33 pr

Research area

Cell biology; Epigenetics and nuclear signaling; Neuroscience

Target protein

CDK1

Recommended dilution

Application Dilution
WB 1: 500 - 1: 2000
IF 1: 200 - 1: 1000
ICC 1: 200 - 1: 1000
ELISA 1: 5000

Images

Immunofluorescence analysis of COS7 cells, using CDK1/CDC2 (Phospho-Thr14) Antibody. The picture on the right is blocked with the phospho peptide.
Western Blot analysis of various cells using Phospho-Cdk1/2/3 (T14) Polyclonal Antibody diluted at 1:2000
Western Blot analysis of COS7 cells using Phospho-Cdk1/2/3 (T14) Polyclonal Antibody diluted at 1:2000
Western blot analysis of lysates from HepG2 cells treated with Forskolin 40nM 30', using CDK1/CDC2 (Phospho-Thr14) Antibody. The lane on the right is blocked with the phospho peptide.

Protocols

References

Customer reviews and Q&As

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Product size

Price

  • 20ul

    $108.00

  • 50ul

    $180.00

  • 100ul

    $275.00

Add to Cart

Lead time: Within one week

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