Crk-L(Phospho Tyr207) Polyclonal Antibody

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Home > Product > Antibody > Crk-L(Phospho Tyr207) Polyclonal Antibody

Crk-L(Phospho Tyr207) Polyclonal Antibody

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Human, Mouse, Rat, Monkey

Applications: WB, IHC-p, IF, ICC, ELISA

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse, Rat, Monkey

Applications: WB, IHC-p, IF, ICC, ELISA

Product overview

Full product name

Crk-like protein

Code

BT-AP08110

Host

Rabbit

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from human CrkL around the phosphorylation site of Tyr207. AA range:173-222

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for 1 year

Background

This gene encodes a protein kinase containing SH2 and SH3 (src homology) domains which has been shown to activate the RAS and JUN kinase signaling pathways and transform fibroblasts in a RAS-dependent fashion. It is a substrate of the BCR-ABL tyrosine kinase, plays a role in fibroblast transformation by BCR-ABL, and may be oncogenic.

Uniprot accession

Human: P46109; Mouse: P47941; Rat: Q5U2U2;

Molecular weight

33777

Gene ID

Human: 1399; Mouse: 12929; Rat: 1.00911e+008

Synonyms

Crk-like protein; CRKL; Crk-like protein

Research area

Cancer; Epigenetics and nuclear signaling; Neuroscience; Signal transduction

Target protein

CRKL

Recommended dilution

Application	Dilution
WB	1: 500 - 1: 2000
IHC-p	1: 100 - 1: 300
IF	1: 200 - 1: 1000
ICC	1: 200 - 1: 1000
ELISA	1: 5000

Images

Immunofluorescence analysis of HUVEC cells, using CrkL (Phospho-Tyr207) Antibody. The picture on the right is blocked with the phospho peptide.

Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4°C overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using CrkL (Phospho-Tyr207) Antibody. The picture on the right is blocked with the phospho peptide.

Western Blot analysis of various cells using Phospho-Crk-L (Y207) Polyclonal Antibody

Western blot analysis of lysates from COS7 cells, using CrkL (Phospho-Tyr207) Antibody. The lane on the right is blocked with the phospho peptide.

Protocols

> Protocol Booklet

References

Customer reviews and Q&As

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Datasheet

Product size

Price

20ul

$108.00
50ul

$180.00
100ul

$275.00

Add to Cart

Lead time: Within one week

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