Cyclin B1(Phospho Ser126) Polyclonal Antibody

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Home > Product > Antibody > Cyclin B1(Phospho Ser126) Polyclonal Antibody

Cyclin B1(Phospho Ser126) Polyclonal Antibody

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Human, Mouse, Rat

Applications: WB, IHC-p, IF, ELISA

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC-p, IF, ELISA

Product overview

Full product name

G2/mitotic-specific cyclin-B1

Code

BT-AP15130

Host

Rabbit

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from human Cyclin B1 around the phosphorylation site of Ser126. AA range:91-140

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for 1 year

Background

The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites.

Uniprot accession

Human: P14635; Mouse: P24860; Rat: P30277;

Molecular weight

48337

Gene ID

Human: 891; Mouse: 268697; Rat: 25203

Synonyms

G2/mitotic-specific cyclin-B1; CCNB1; CCNB; G2/mitotic-specific cyclin-B1

Research area

Cancer; Cell biology; Epigenetics and nuclear signaling

Target protein

CCNB1

Recommended dilution

Application	Dilution
WB	1: 500 - 1: 2000
IHC-p	1: 100 - 1: 300
ELISA	1: 5000

Images

Immunohistochemical analysis of paraffin-embedded Human lung cancer. Antibody was diluted at 1:100(4°C overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using Cyclin B1 (Phospho-Ser126) Antibody. The picture on the right is blocked with the phospho peptide.

Western Blot analysis of various cells using Phospho-Cyclin B1 (S126) Polyclonal Antibody

Western blot analysis of lysates from NIH/3T3 cells treated with EGF 200ng/ml 15', using Cyclin B1 (Phospho-Ser126) Antibody. The lane on the right is blocked with the phospho peptide.

Protocols

> Protocol Booklet

References

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Datasheet

Product size

Price

20ul

$108.00
50ul

$180.00
100ul

$275.00

Add to Cart

Lead time: Within one week

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