DARPP-32(Phospho Thr75) Polyclonal Antibody

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Home > Product > Antibody > DARPP-32(Phospho Thr75) Polyclonal Antibody

DARPP-32(Phospho Thr75) Polyclonal Antibody

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Human, Mouse, Rat, Monkey

Applications: WB, IHC-p, IF, ELISA

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse, Rat, Monkey

Applications: WB, IHC-p, IF, ELISA

Product overview

Full product name

Protein phosphatase 1 regulatory subunit 1B

Code

BT-AP15184

Host

Rabbit

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from human DARPP-32 around the phosphorylation site of Thr75. AA range:41-90

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for 1 year

Background

This gene encodes a bifunctional signal transduction molecule. Dopaminergic and glutamatergic receptor stimulation regulates its phosphorylation and function as a kinase or phosphatase inhibitor. As a target for dopamine, this gene may serve as a therapeutic target for neurologic and psychiatric disorders. Multiple transcript variants encoding different isoforms have been found for this gene.

Uniprot accession

Human: Q9UD71; Mouse: Q60829; Rat: Q6J4I0;

Molecular weight

22963

Gene ID

Human: 84152; Mouse: 19049; Rat: 360616

Synonyms

Protein phosphatase 1 regulatory subunit 1B; PPP1R1B; DARPP32; Protein phosphatase 1 regulatory subunit 1B; DARPP-32; Dopamine- and cAMP-regulated neuronal phosphoprotein

Research area

Metabolism; Neuroscience; Signal transduction

Target protein

PPP1R1B

Recommended dilution

Application	Dilution
WB	1: 500 - 1: 2000
IHC-p	1: 100 - 1: 300
ELISA	1: 40000

Images

Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4°C overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4°C overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using DARPP-32 (Phospho-Thr75) Antibody. The picture on the right is blocked with the phospho peptide.

Western Blot analysis of various cells using Phospho-DARPP-32 (T75) Polyclonal Antibody diluted at 1:500

Western blot analysis of lysates from COS7 cells treated with Forskolin 40nM 30', using DARPP-32 (Phospho-Thr75) Antibody. The lane on the right is blocked with the phospho peptide.

Protocols

> Protocol Booklet

References

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Datasheet

Product size

Price

20ul

$108.00
50ul

$180.00
100ul

$275.00

Add to Cart

Lead time: Within one week

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