Emt(Phospho Tyr512) Polyclonal Antibody

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Home > Product > Antibody > Emt(Phospho Tyr512) Polyclonal Antibody

Emt(Phospho Tyr512) Polyclonal Antibody

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Human, Mouse

Applications: WB, IHC-p, IF, ELISA

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, IHC-p, IF, ELISA

Product overview

Full product name

Tyrosine-protein kinase ITK/TSK

Code

BT-AP15397

Host

Rabbit

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from human ITK around the phosphorylation site of Tyr512. AA range:478-527

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for 1 year

Background

This gene encodes an intracellular tyrosine kinase expressed in T-cells. The protein contains both SH2 and SH3 domains which are often found in intracellular kinases. It is thought to play a role in T-cell proliferation and differentiation.

Uniprot accession

Human: Q08881; Mouse: Q03526;

Molecular weight

71831

Gene ID

Human: 3702; Mouse: 16428

Synonyms

Tyrosine-protein kinase ITK/TSK; ITK; EMT; LYK; Tyrosine-protein kinase ITK/TSK; Interleukin-2-inducible T-cell kinase; IL-2-inducible T-cell kinase; Kinase EMT; T-cell-specific kinase; Tyrosine-protein kinase Lyk

Research area

Signal transduction

Target protein

ITK

Recommended dilution

Application	Dilution
WB	1: 500 - 1: 2000
IHC-p	1: 100 - 1: 300
ELISA	1: 5000

Images

Immunohistochemical analysis of paraffin-embedded Human testis. Antibody was diluted at 1:100(4°C overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Western blot analysis of 293 using p-Emt (Y512) antibody.

Protocols

> Protocol Booklet

References

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Datasheet

Product size

Price

20ul

$108.00
50ul

$180.00
100ul

$275.00

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Lead time: Within one week

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