IRF-3(Phospho Ser385) Polyclonal Antibody

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Home > Product > Antibody > IRF-3(Phospho Ser385) Polyclonal Antibody

IRF-3(Phospho Ser385) Polyclonal Antibody

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Human, Rat, Mouse

Applications: WB, IHC-p, IF, ICC, ELISA

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Rat, Mouse

Applications: WB, IHC-p, IF, ICC, ELISA

Product overview

Full product name

Interferon regulatory factor 3

Code

BT-AP10486

Host

Rabbit

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from human IRF-3 around the phosphorylation site of Ser385. AA range:351-400

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for 1 year

Background

This gene encodes a member of the interferon regulatory transcription factor (IRF) family. The encoded protein is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Uniprot accession

Human: Q14653; Mouse: P70671;

Molecular weight

47219

Gene ID

Human: 3661

Synonyms

Interferon regulatory factor 3; IRF3; Interferon regulatory factor 3; IRF-3

Research area

Epigenetics and nuclear signaling; Immunology ; Microbiology

Target protein

IRF3

Recommended dilution

Application	Dilution
WB	1: 500 - 1: 2000
IHC-p	1: 100 - 1: 300
IF	1: 200 - 1: 1000
ICC	1: 200 - 1: 1000
ELISA	1: 10000

Images

Immunofluorescence analysis of Hela cell. IRF-3 (phospho Ser385) Polyclonal Antibody(Red) was diluted at 1:200(4°C overnight). COX IV Monoclonal Antibody(6C8)(Green) was diluted at 1:200(4°C overnight).

Immunofluorescence analysis of HeLa cells, using IRF-3 (Phospho-Ser385) Antibody. The picture on the right is blocked with the phospho peptide.

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using IRF-3 (Phospho-Ser385) Antibody. The picture on the right is blocked with the phospho peptide.

Western Blot analysis of 293 cells using Phospho-IRF-3 (S385) Polyclonal Antibody

Western blot analysis of lysates from HT29 cells treated with INSULIN 0.01U/ML 15', using IRF-3 (Phospho-Ser385) Antibody. The lane on the right is blocked with the phospho peptide.

Protocols

> Protocol Booklet

References

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Datasheet

Product size

Price

20ul

$108.00
50ul

$180.00
100ul

$275.00

Add to Cart

Lead time: Within one week

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