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Home > Product > Antibody > MBP-Tag Polyclonal Antibody

MBP-Tag Polyclonal Antibody

MBP-Tag Polyclonal Antibody

Product Summary

Size: 100ul,50uL,20ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Species independent

Applications: WB

Product Summary

Size: 100ul,50uL,20ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Species independent

Applications: WB

Product overview

Code

BT-AP11161

Host

Rabbit

Isotype

IgG

Size

100ul,50uL,20ul

Clonality

Polyclonal

Immunogen

Recombinant Protein of MBP-Tag

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen.

Formulation

PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

Storage

-20°C for 1 year

Background

Maltose binding protein (MBP) is the 370 amino acid product of the E.coli mal E gene. MBP is a useful affinity tag that can increase the expression level and solubility of the resulting tagged protein. The MBP tag also promotes proper folding of the attached protein. Plasmid vectors have been constructed utilizing the MBP domain that allow the synthesis of high levels of MBP-fusion proteins that can be purified in a one step procedure by affinity chromatography cross linked amylose resin. Once bound to amylose, the MBP protein can then be separated from the target protein by cleavage by coagulation Factor Xa at a specific four residue site. Alternatively, the intact fusion protein can be specifically eluted from the resin by the addition of excess free maltose.

Research area

Tags & cell markers

Recommended dilution

Application Dilution
WB 1: 5000

Protocols

References

Customer reviews and Q&As

No reviews
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Product size

Price

  • 100ul

    $275.00

  • 50uL

    $180.00

  • 20ul

    $108.00

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Lead time: Within one week

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