NIFK(Phospho Thr234) Polyclonal Antibody

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Home > Product > Antibody > NIFK(Phospho Thr234) Polyclonal Antibody

NIFK(Phospho Thr234) Polyclonal Antibody

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Human, Mouse

Applications: IHC-p, IF, ICC, ELISA

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse

Applications: IHC-p, IF, ICC, ELISA

Product overview

Full product name

MKI67 FHA domain-interacting nucleolar phosphoprotein

Code

BT-AP11872

Host

Rabbit

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from human NIFK around the phosphorylation site of Thr234. AA range:200-249

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for 1 year

Background

This gene encodes a protein that interacts with the forkhead-associated domain of the Ki-67 antigen. The encoded protein may bind RNA and may play a role in mitosis and cell cycle progression. Multiple pseudogenes exist on chromosomes 5, 10, 12, 15, and 19.

Uniprot accession

Human: Q9BYG3; Mouse: Q91VE6;

Molecular weight

34222

Gene ID

Human: 84365; Mouse: 67949

Synonyms

MKI67 FHA domain-interacting nucleolar phosphoprotein; MKI67IP; NIFK; NOPP34; MKI67 FHA domain-interacting nucleolar phosphoprotein; Nucleolar phosphoprotein Nopp34; Nucleolar protein interacting with the FHA domain of pKI-67; hNIFK

Research area

Cell biology

Target protein

MKI67IP

Recommended dilution

Application	Dilution
IHC-p	1: 100 - 1: 300
IF	1: 200 - 1: 1000
ICC	1: 200 - 1: 1000
ELISA	1: 40000

Images

Immunofluorescence analysis of HUVEC cells, using NIFK (Phospho-Thr234) Antibody. The picture on the right is blocked with the phospho peptide.

Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4°C overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Immunohistochemistry analysis of paraffin-embedded human brain, using NIFK (Phospho-Thr234) Antibody. The picture on the right is blocked with the phospho peptide.

Protocols

> Protocol Booklet

References

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Datasheet

Product size

Price

20ul

$108.00
50ul

$180.00
100ul

$275.00

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Lead time: Within one week

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