Syk(Phospho Tyr348) Polyclonal Antibody

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Home > Product > Antibody > Syk(Phospho Tyr348) Polyclonal Antibody

Syk(Phospho Tyr348) Polyclonal Antibody

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Human, Mouse, Rat, Monkey

Applications: WB, IHC-p, IF, ELISA

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse, Rat, Monkey

Applications: WB, IHC-p, IF, ELISA

Product overview

Full product name

Tyrosine-protein kinase SYK

Code

BT-AP14610

Host

Rabbit

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from human SYK around the phosphorylation site of Tyr348. AA range:314-363

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for 1 year

Background

This gene encodes a member of the family of non-receptor type Tyr protein kinases. This protein is widely expressed in hematopoietic cells and is involved in coupling activated immunoreceptors to downstream signaling events that mediate diverse cellular responses, including proliferation, differentiation, and phagocytosis. It is thought to be a modulator of epithelial cell growth and a potential tumour suppressor in human breast carcinomas. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Uniprot accession

Human: P43405; Mouse: P48025; Rat: Q64725;

Molecular weight

72066

Gene ID

Human: 6850; Mouse: 20963; Rat: 25155

Synonyms

Tyrosine-protein kinase SYK; SYK; Tyrosine-protein kinase SYK; Spleen tyrosine kinase; p72-Syk

Research area

Cardiovascular; Signal transduction; Immunology

Target protein

SYK

Recommended dilution

Application	Dilution
WB	1: 500 - 1: 2000
IHC-p	1: 100 - 1: 300
ELISA	1: 10000

Images

Immunohistochemical analysis of paraffin-embedded Human lung cancer. Antibody was diluted at 1:100(4°C overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using SYK (Phospho-Tyr348) Antibody. The picture on the right is blocked with the phospho peptide.

Western Blot analysis of various cells using Phospho-Syk (Y348) Polyclonal Antibody

Western Blot analysis of 3T3 cells using Phospho-Syk (Y348) Polyclonal Antibody

Western blot analysis of lysates from COS7 cells treated with EGF 200ng/ml 30', using SYK (Phospho-Tyr348) Antibody. The lane on the right is blocked with the phospho peptide.

Western blot analysis of lysates from 1) 3T3, 2) HT29, 3) HepG2 cells, (Green) primary antibody was diluted at 1:1000, 4°C overnight, secondary antibody was diluted at 1:10000, 37°C 1hour. (Red) Actin β Monoclonal Antibody(5B7) antibody was diluted at 1:5000 as loading control, 4°C overnight,secondary was diluted at 1:10000, 37°C 1hour.

Protocols

> Protocol Booklet

References

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Datasheet

Product size

Price

20ul

$108.00
50ul

$180.00
100ul

$275.00

Add to Cart

Lead time: Within one week

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