YAP(Phospho Ser127) Polyclonal Antibody

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Blood coagulation
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Signal transduction
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Epigenetics and nuclear signaling
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Home > Product > Antibody > YAP(Phospho Ser127) Polyclonal Antibody

YAP(Phospho Ser127) Polyclonal Antibody

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Human, Mouse, Rat, Monkey

Applications: IF, ICC, WB, IHC-p, ELISA

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse, Rat, Monkey

Applications: IF, ICC, WB, IHC-p, ELISA

Product overview

Full product name

Yorkie homolog

Code

BT-AP15585

Host

Rabbit

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from human YAP around the phosphorylation site of Ser127. AA range:93-142

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for 1 year

Background

This gene encodes a downstream nuclear effector of the Hippo signaling pathway which is involved in development, growth, repair, and homeostasis. This gene is known to play a role in the development and progression of multiple cancers as a transcriptional regulator of this signaling pathway and may function as a potential target for cancer treatment. Alternative splicing results in multiple transcript variants encoding different isoforms.

Uniprot accession

Human: P46937; Mouse: P46938; Rat: Q2EJA0;

Molecular weight

48755

Gene ID

Human: 10413; Mouse: 22601; Rat: 363014

Synonyms

Yorkie homolog; YAP1; YAP65; Yorkie homolog; 65 kDa Yes-associated protein; YAP65

Research area

Epigenetics and nuclear signaling; Cancer; Signal transduction

Target protein

YAP1

Recommended dilution

Application	Dilution
WB	1: 500 - 1: 2000
IHC-p	1: 100 - 1: 300
IF	1: 50 - 1: 200
ELISA	1: 40000

Images

Immunofluorescence analysis of Hela cell. YAP (phospho Ser127) Polyclonal Antibody(Red) was diluted at 1:200(4°C overnight). β-Tubulin Monoclonal Antibody(5G3)(Green) was diluted at 1:200(4°C overnight).

Immunofluorescence analysis of rat-heart tissue. 1,YAP (phospho Ser127) Polyclonal Antibody(Red) was diluted at 1:200(4°C overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunohistochemistry analysis of paraffin-embedded human heart , using YAP (Phospho-Ser127) Antibody. The picture on the right is blocked with the phospho peptide.

Western Blot analysis of various cells using Phospho-YAP (S127) Polyclonal Antibody diluted at 1:500

Western Blot analysis of MCF7 cells using Phospho-YAP (S127) Polyclonal Antibody diluted at 1:500

Western blot analysis of lysates from COS7 cells treated with HU 2nM 24h, using YAP (Phospho-Ser127) Antibody. The lane on the right is blocked with the phospho peptide.

Western blot analysis of lysates from 1)MCF-7 , 2)PC-3, 3) KB, 4) HELA cells, (Green) primary antibody was diluted at 1:1000, 4°C overnight, secondary antibody was diluted at 1:10000, 37°C 1hour. (Red) Actin β Monoclonal Antibody(5B7) was diluted at 1:5000 as loading control, 4°C overnight,secondary antibody was diluted at 1:10000, 37°C 1hour.

Protocols

> Protocol Booklet

References

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Datasheet

Product size

Price

20ul

$108.00
50ul

$180.00
100ul

$275.00

Add to Cart

Lead time: Within one week

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