ACAT-1 Polyclonal Antibody

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Home > Product > Antibody > ACAT-1 Polyclonal Antibody

ACAT-1 Polyclonal Antibody

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Human, Mouse, Rat

Applications: WB, IHC-p, ELISA

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC-p, ELISA

Product overview

Full product name

ACAT-1 Antibody

Code

BT-AP00142

Host

Rabbit

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from human ACAT1. AA range:221-270

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for one year

Background

ACAT1 encodes a mitochondrially localized enzyme (acetyl-CoA acetyltransferase 1) that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in ACAT1 are associated with 3-ketothiolase deficiency, an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone.

Uniprot accession

Human: P24752; Mouse: Q8QZT1; Rat: P17764;

Molecular weight

45200

Gene ID

Human: 38; Mouse: 110446; Rat: 25014

Synonyms

ACAT1; ACAT; MAT; Acetyl-CoA acetyltransferase; mitochondrial; Acetoacetyl-CoA thiolase; T2

Research area

Tags & Cell Markers; Signal Transduction

Target protein

ACAT1

Recommended dilution

Application	Dilution
WB	1: 500 - 1: 2000
IHC	1: 100 - 1: 300
ELISA	1: 40000

Images

Western blot analysis of the lysates from HepG2 cells using ACAT1 antibody.

Western Blot analysis of various cells using ACAT-1 Polyclonal Antibody

Western blot analysis of lysates from HepG2, Jurkat, 293, and A549 cells, using ACAT1 Antibody. The lane on the right is blocked with the synthesized peptide.

Western Blot analysis of A549 cells using ACAT-1 Polyclonal Antibody

Protocols

> Protocol Booklet

References

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Datasheet

Product size

Price

20ul

$108.00
50ul

$180.00
100ul

$275.00

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Lead time: Within one week

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