AP-2Gamma Polyclonal Antibody

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Home > Product > Antibody > AP-2Gamma Polyclonal Antibody

AP-2Gamma Polyclonal Antibody

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Human, Mouse, Rat

Applications: WB, IHC-p, ELISA

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC-p, ELISA

Product overview

Full product name

AP-2gamma Antibody

Code

BT-AP00500

Host

Rabbit

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from human AP2C. AA range:401-450

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for one year

Background

The protein encoded by TFAP2C is a sequence-specific DNA-binding transcription factor involved in the activation of several developmental genes. The encoded protein (transcription factor AP-2 gamma) can act as either a homodimer or heterodimer with other family members and is induced during retinoic acid-mediated differentiation. It plays a role in the development of the eyes, face, body wall, limbs, and neural tube.

Uniprot accession

Human: Q92754; Mouse: Q61312;

Molecular weight

49177

Gene ID

Human: 7022; Mouse: 21420

Synonyms

TFAP2C; Transcription factor AP-2 gamma; AP2-gamma; Activating enhancer-binding protein 2 gamma; Transcription factor ERF-1

Research area

Cell Biology

Target protein

TFAP2C

Recommended dilution

Application	Dilution
WB	1: 500 - 1: 2000
IHC	1: 100 - 1: 300
ELISA	1: 40000

Images

Western Blot analysis of various cells using AP-2γ Polyclonal Antibody cells nucleus.

Immunohistochemical analysis of paraffin-embedded Human breast. 1, Antibody was diluted at 1:200(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).

Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Western blot analysis of lysates from HepG2 and HUVEC cells, using AP2C Antibody. The lane on the right is blocked with the synthesized peptide.

Western blot analysis of the lysates from K562 cells using AP2C antibody.

Protocols

> Protocol Booklet

References

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Datasheet

Product size

Price

20ul

$108.00
50ul

$180.00
100ul

$275.00

Add to Cart

Lead time: Within one week

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