A-Raf Polyclonal Antibody

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Home > Product > Antibody > A-Raf Polyclonal Antibody

A-Raf Polyclonal Antibody

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Human, Mouse, Rat

Applications: WB, IHC-p, ELISA

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC-p, ELISA

Product overview

Full product name

A-Raf Antibody

Code

BT-AP00577

Host

Rabbit

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from human A-RAF. AA range:276-325

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for one year

Background

This proto-oncogene belongs to the RAF subfamily of the Ser/Thr protein kinase family, and maybe involved in cell growth and development. Alternatively spliced transcript variants encoding different isoforms have been found for ARAF.

Uniprot accession

Human: P10398; Mouse: P04627; Rat: P14056;

Molecular weight

67585

Gene ID

Human: 369; Mouse: 11836; Rat: 64363

Synonyms

ARAF; ARAF1; PKS; PKS2; Serine/threonine-protein kinase A-Raf; Proto-oncogene A-Raf; Proto-oncogene A-Raf-1; Proto-oncogene Pks

Research area

Neuroscience

Target protein

ARAF

Recommended dilution

Application	Dilution
WB	1: 500 - 1: 2000
IHC	1: 100 - 1: 300
ELISA	1: 20000

Images

Western Blot analysis of various cells using A-Raf Polyclonal Antibody

Western blot analysis of lysates from HeLa cells, treated with PMA 125ng/ml 30', using A-RAF Antibody. The lane on the right is blocked with the synthesized peptide.

Western blot analysis in MCF-7 cells transfected with siA-Raf. Cancer Letters 355 (2014) 288–296

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using A-RAF Antibody. The picture on the right is blocked with the synthesized peptide.

Protocols

> Protocol Booklet

References

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Datasheet

Product size

Price

20ul

$108.00
50ul

$180.00
100ul

$275.00

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Lead time: Within one week

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