Bcl-2 Polyclonal Antibody

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Home > Product > Antibody > Bcl-2 Polyclonal Antibody

Bcl-2 Polyclonal Antibody

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Mouse, Rat

Applications: WB, IHC-p, ELISA

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Mouse, Rat

Applications: WB, IHC-p, ELISA

Product overview

Full product name

Bcl-2 Antibody

Code

BT-AP00860

Host

Rabbit

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Polyclonal

Immunogen

Synthesized peptide derived from Bcl-2 . at AA range: 10-90

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for one year

Background

BCL2 (BCL2, apoptosis regulator) encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants.

Uniprot accession

Human: P10415; Mouse: P10417;

Molecular weight

26266

Gene ID

Human: 596

Synonyms

B-cell CLL/lymphoma 2

Research area

Cell Biology

Target protein

BCL2

Recommended dilution

Application	Dilution
WB	1: 500 - 2000
IHC	1:50-300
ELISA	1: 10000 - 20000

Images

Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1,Bcl-2 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Mouse-lung tissue. 1,Bcl-2 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.

Western Blot analysis of mouse-spleen cells using Bcl-2 Polyclonal Antibody diluted at 1:500. Secondary antibody was diluted at 1:20000

Protocols

> Protocol Booklet

References

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Datasheet

Product size

Price

20ul

$108.00
50ul

$180.00
100ul

$275.00

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Lead time: Within one week

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