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Home > Product > Antibody > COL5A3 Polyclonal Antibody

COL5A3 Polyclonal Antibody

COL5A3 Polyclonal Antibody

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Human

Applications: WB, IHC-p, IF, ELISA

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Applications: WB, IHC-p, IF, ELISA

Product overview

Full product name

COL5A3 Antibody

Code

BT-AP02091

Host

Rabbit

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from human Collagen V alpha3. AA range:221-270

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for one year

Background

COL5A3 encodes an alpha chain for one of the low abundance fibrillar collagens. Fibrillar collagen molecules are trimers that can be composed of one or more types of alpha chains. Type V collagen is found in tissues containing type I collagen and appears to regulate the assembly of heterotypic fibers composed of both type I and type V collagen. This gene product is closely related to type XI collagen and it is possible that the collagen chains of types V and XI constitute a single collagen type with tissue-specific chain combinations. Mutations in COL5A3 are thought to be responsible for the symptoms of a subset of patients with Ehlers-Danlos syndrome type III. Messages of several sizes can be detected in northern blots but sequence information cannot confirm the identity of the shorter messages.

Uniprot accession

Molecular weight

172052

Gene ID

Human: 50509

Synonyms

COL5A3; Collagen alpha-3(V) chain

Research area

Others

Target protein

COL5A3

Recommended dilution

Application Dilution
WB 1: 500 - 1: 2000
IHC 1: 100 - 1: 300
IF 1: 200 - 1: 1000
ELISA 1: 20000

Images

Western Blot analysis of various cells using COL5A3 Polyclonal Antibody
Immunofluorescence analysis of HeLa cells, using Collagen V alpha3 Antibody. The picture on the right is blocked with the synthesized peptide.
Western Blot analysis of K562 cells using COL5A3 Polyclonal Antibody
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Collagen V alpha3 Antibody. The picture on the right is blocked with the synthesized peptide.

Protocols

References

Customer reviews and Q&As

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Product size

Price

  • 20ul

    $108.00

  • 50ul

    $180.00

  • 100ul

    $275.00

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Lead time: Within one week

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