Eg5 Polyclonal Antibody

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Home > Product > Antibody > Eg5 Polyclonal Antibody

Eg5 Polyclonal Antibody

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Human

Applications: WB, IHC-p, IF, ELISA

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Applications: WB, IHC-p, IF, ELISA

Product overview

Full product name

Eg5 Antibody

Code

BT-AP02856

Host

Rabbit

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from human KIF11/Eg5. AA range:892-941

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for one year

Background

KIF11 encodes a motor protein that belongs to the kinesin-like protein family. Members of this protein family are known to be involved in various kinds of spindle dynamics. The function of KIF11 product includes chromosome positioning, centrosome separation and establishing a bipolar spindle during cell mitosis.

Uniprot accession

Human: P52732; Mouse: Q6P9P6;

Molecular weight

119159

Gene ID

Human: 3832

Synonyms

KIF11; EG5; KNSL1; TRIP5; Kinesin-like protein KIF11; Kinesin-like protein 1; Kinesin-like spindle protein HKSP; Kinesin-related motor protein Eg5; Thyroid receptor-interacting protein 5; TR-interacti

Research area

Signal Transduction

Target protein

KIF11

Recommended dilution

Application	Dilution
WB	1: 500 - 1: 2000
IHC	1: 100 - 1: 300
IF	1: 200 - 1: 1000
ELISA	1: 20000

Images

Immunohistochemical analysis of paraffin-embedded Human lung cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Western Blot analysis of various cells using Eg5 Polyclonal Antibody cells nucleus.

Western Blot analysis of COLO205 cells using Eg5 Polyclonal Antibody cells nucleus.

Western blot analysis of lysates from Jurkat, HepG2, and COS cells, using KIF11/Eg5 Antibody. The lane on the right is blocked with the synthesized peptide.

Immunofluorescence analysis of HeLa cells, using KIF11/Eg5 Antibody. The picture on the right is blocked with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using KIF11/Eg5 Antibody. The picture on the right is blocked with the synthesized peptide.

Protocols

> Protocol Booklet

References

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Datasheet

Product size

Price

20ul

$108.00
50ul

$180.00
100ul

$275.00

Add to Cart

Lead time: Within one week

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