Enolase Polyclonal Antibody

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Home > Product > Antibody > Enolase Polyclonal Antibody

Enolase Polyclonal Antibody

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Human, Mouse, Rat

Applications: WB, IHC-p, IF, ELISA

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC-p, IF, ELISA

Product overview

Full product name

Enolase Antibody

Code

BT-AP02967

Host

Rabbit

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from human NSE. AA range:371-420

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for one year

Background

ENO2 encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates.

Uniprot accession

Human: P09104; Mouse: P17183; Rat: P07323;

Molecular weight

47269

Gene ID

Human: 2026; Mouse: 13807; Rat: 1.00912e+008

Synonyms

ENO2; Gamma-enolase; 2-phospho-D-glycerate hydro-lyase; Enolase 2; Neural enolase; Neuron-specific enolase; NSE

Research area

Neuroscience

Target protein

ENO2

Recommended dilution

Application	Dilution
WB	1: 500 - 1: 2000
IHC	1: 100 - 1: 300
IF	1: 200 - 1: 1000
ELISA	1: 10000

Images

Western Blot analysis of various cells using Enolase Polyclonal Antibody diluted at 1:2000

Western blot analysis of lysates from HepG2 cells, using NSE Antibody. The lane on the right is blocked with the synthesized peptide.

Immunofluorescence analysis of A549 cells, using NSE Antibody. The picture on the right is blocked with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using NSE Antibody. The picture on the right is blocked with the synthesized peptide.

Immunofluorescence analysis of Hela cell. 1,Enolase Polyclonal Antibody(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 488 was diluted at 1:1000(room temperature, 50min). 3 DAPI(blue) 10min.

Western Blot analysis of HepG2 cells using Enolase Polyclonal Antibody diluted at 1:2000

Protocols

> Protocol Booklet

References

Customer reviews and Q&As

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Datasheet

Product size

Price

20ul

$108.00
50ul

$180.00
100ul

$275.00

Add to Cart

Lead time: Within one week

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