Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate-limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. GCLC encodes Glutamate--cysteine ligase catalytic subunit, while the regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Mutations at GCLC have been associated with hemolytic anemia due to deficiency of gamma-glutamylcysteine synthetase and susceptibility to myocardial infarction.