GFAP Polyclonal Antibody

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Home > Product > Antibody > GFAP Polyclonal Antibody

GFAP Polyclonal Antibody

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Human

Applications: WB, IHC-p, IF, ELISA

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Applications: WB, IHC-p, IF, ELISA

Product overview

Full product name

GFAP Antibody

Code

BT-AP03549

Host

Rabbit

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from human GFAP. AA range:11-60

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for one year

Background

GFAP encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Uniprot accession

Human: P14136; Mouse: P03995;

Molecular weight

49880

Gene ID

Human: 2670

Synonyms

GFAP; Glial fibrillary acidic protein; GFAP

Research area

Neuroscience

Target protein

GFAP

Recommended dilution

Application	Dilution
WB	1: 500 - 1: 2000
IHC	1: 100 - 1: 300
IF	1: 200 - 1: 1000
ELISA	1: 5000

Images

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using GFAP Antibody. The picture on the right is blocked with the synthesized peptide.

Immunofluorescence analysis of Hela cell. 1,GFAP Polyclonal Antibody(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 488 was diluted at 1:1000(room temperature, 50min). 3 DAPI(blue) 10min.

Western Blot analysis of RAW using GFAP Polyclonal Antibody diluted at 1:2000

Western blot analysis of lysates from COLO205 cells, using GFAP Antibody. The lane on the right is blocked with the synthesized peptide.

Western Blot analysis of RAT-MUSCLE cells using GFAP Polyclonal Antibody diluted at 1:2000

Immunofluorescence analysis of COS7 cells, using GFAP Antibody. The picture on the right is blocked with the synthesized peptide.

Protocols

> Protocol Booklet

References

Customer reviews and Q&As

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Datasheet

Product size

Price

20ul

$108.00
50ul

$180.00
100ul

$275.00

Add to Cart

Lead time: Within one week

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