Histone H1 Polyclonal Antibody

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Home > Product > Antibody > Histone H1 Polyclonal Antibody

Histone H1 Polyclonal Antibody

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Human, Mouse

Applications: WB, IHC-p, IF, ELISA

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, IHC-p, IF, ELISA

Product overview

Full product name

Histone H1 Antibody

Code

BT-AP03994

Host

Rabbit

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from human Histone H1. AA range:1-50

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for one year

Background

Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. HIST1H1B is intronless and encodes a replication-dependent histone that is a member of the histone H1 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. HIST1H1B is found in the small histone gene cluster on chromosome 6p22-p21.3.

Uniprot accession

Human: P16401/P16402/P10412;

Molecular weight

22580

Gene ID

Human: 3009/3007/3008; Mouse: 56702/14957/50709

Synonyms

HIST1H1B; H1F5; Histone H1.5; Histone H1a; Histone H1b; Histone H1s-3; HIST1H1D; H1F3; Histone H1.3; Histone H1c; Histone H1s-2; HIST1H1E; H1F4; Histone H1.4; Histone H1b; Histone H1s-4

Research area

Others

Target protein

HIST1H1B

Recommended dilution

Application	Dilution
WB	1: 500 - 1: 2000
IHC	1: 100 - 1: 300
IF	1: 200 - 1: 1000
ELISA	1: 20000

Images

Immunofluorescence analysis of Hela cell. 1,Histone H1 Polyclonal Antibody(red) was diluted at 1:200(4° overnight). α-SMA Monoclonal Antibody(6A12)(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000(room temperature, 50min).

Western Blot analysis of various cells using Histone H1 Polyclonal Antibody diluted at 1:1000 cells nucleus.

Western blot analysis of lysates from COLO cells, using Histone H1 Antibody. The lane on the right is blocked with the synthesized peptide.

Western Blot analysis of 3T3 cells using Histone H1 Polyclonal Antibody diluted at 1:1000 cells nucleus.

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using Histone H1 Antibody. The picture on the right is blocked with the synthesized peptide.

Immunofluorescence analysis of HUVEC cells, using Histone H1 Antibody. The picture on the right is blocked with the synthesized peptide.

Protocols

> Protocol Booklet

References

Customer reviews and Q&As

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Datasheet

Product size

Price

20ul

$108.00
50ul

$180.00
100ul

$275.00

Add to Cart

Lead time: Within one week

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