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Home > Product > Antibody > Histone H2B Polyclonal Antibody

Histone H2B Polyclonal Antibody

Histone H2B Polyclonal Antibody

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Human, Mouse, Monkey

Applications: WB, IHC-p, IF, ELISA

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse, Monkey

Applications: WB, IHC-p, IF, ELISA

Product overview

Full product name

Histone H2B Antibody

Code

BT-AP04032

Host

Rabbit

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from human Histone H2B. AA range:10-59

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for one year

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. HIST1H2BA is intronless and encodes a replication-dependent histone that is a testis/sperm-specific member of the histone H2B family. Transcripts from HIST1H2BA contain a palindromic termination element.

Uniprot accession

Molecular weight

14167/13950/13906

Gene ID

Human: 255626/3018/3017/8339/8343/8344/8346/8347; Mouse: 319177/319178/319179; Rat: 24829

Synonyms

H2BFS; Histone H2B type F-S; Histone H2B.s; H2B/s

Research area

Others

Target protein

H2BFS

Recommended dilution

Application Dilution
WB 1: 500 - 1: 2000
IHC 1: 100 - 1: 300
IF 1: 200 - 1: 1000
ELISA 1: 5000

Images

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Histone H2B Antibody. The picture on the right is blocked with the synthesized peptide.
Immunofluorescence analysis of HeLa cells, using Histone H2B Antibody. The picture on the right is blocked with the synthesized peptide.
Western Blot analysis of COS7 cells using Histone H2B Polyclonal Antibody diluted at 1:1000 cells nucleus.
Western Blot analysis of various cells using Histone H2B Polyclonal Antibody diluted at 1:1000 cells nucleus.
Western blot analysis of lysates from COS7 cells, treated with TSA 400nM 24h, using Histone H2B Antibody. The lane on the right is blocked with the synthesized peptide.

Protocols

References

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Product size

Price

  • 20ul

    $108.00

  • 50ul

    $180.00

  • 100ul

    $275.00

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Lead time: Within one week

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