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Home > Product > Antibody > Histone H3.3 Polyclonal Antibody

Histone H3.3 Polyclonal Antibody

Histone H3.3 Polyclonal Antibody

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Human, Mouse, Rat

Applications: WB, IHC-p, IF, ELISA

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC-p, IF, ELISA

Product overview

Full product name

Histone H3.3 Antibody

Code

BT-AP04098

Host

Rabbit

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from human Histone H3.3. AA range:16-65

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for one year

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. H3F3A contains introns and its mRNA is polyadenylated, unlike most histone genes. Histone H3. encoded is a replication-independent member of the histone H3 family.

Uniprot accession

Molecular weight

15328

Gene ID

Human: 3020/3021; Mouse: 15078; Rat: 1.00362e+008

Synonyms

H3F3A; H3.3A; H3F3; PP781; H3F3B; H3.3B; Histone H3.3

Research area

Epigenetics and Nuclear Signaling

Target protein

H3F3A

Recommended dilution

Application Dilution
IHC 1: 100 - 1: 300
IF 1: 200 - 1: 1000
ELISA 1: 5000

Images

Immunofluorescence analysis of Hela cell. 1,Histone H3.3 Polyclonal Antibody(red) was diluted at 1:200(4° overnight). GAPDH Monoclonal Antibody(2B8)(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000(room temperature, 50min).
Western Blot analysis of Hela cells using Histone H3.3 Polyclonal Antibody diluted at 1:1000 cells nucleus.
Immunofluorescence analysis of HeLa cells, using Histone H3.3 Antibody. The picture on the right is blocked with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human brain, using Histone H3.3 Antibody. The picture on the right is blocked with the synthesized peptide.
Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Protocols

References

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Product size

Price

  • 20ul

    $108.00

  • 50ul

    $180.00

  • 100ul

    $275.00

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Lead time: Within one week

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